other:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
Virus:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit-anti-GABA A receptor alpha1 Alomone Labs Cat#AGA001; RRID: AB_2756618 Mouse-anti-GAPDH ThermoFisher MA5-15738; RRID: AB_2537652 Rat-anti-a-tubulin Abcam Cat#ab6160; RRID: AB_305328 Rabbit-anti-GFAP Sigma Cat#SAB5600060 Rabbit-anti-Connexin-43 Abcam Cat#ab11370; RRID: AB_297976 Mouse-anti-c-fos Abcam Cat#ab208942; RRID: AB_2747772 Rabbit-anti-H3K9me3 Abcam Catab8898; RRID: AB_306848 Bacterial and virus strains AAV1-CMV-EGFP Vectorbiolab Cat#AAV-7002 AAV1-CMV-VEGF-C Vectorbiolab Cat#AAV-275994 AAV9-GAD67-InhiPre Lee et al.47 N/A AAV9-VEGFR3(Ig1-3)-Fc Song et al.34 N/A AAV9-VEGFR3(Ig4-7)-Fc Song et al.34 N/A Chemicals, peptides, and recombinant proteins 1200 mg/kg PLX5622-containing food chow Plexxikon Cat#D11100404I Control food chow for PLX5622 treatment Plexxikon Cat#D10001i Mouse recombininant IL-6 R&D systems Cat#40-6ML-200CF Ovalbumin Hooke Laboratories Inc. Cat#DSD0142 Soluble gp130-Fc R&D systems Cat#468-MG-100 Sterile artificial CSF Tocris Cat#3525-25ML Heparin sodium, porcine 1000 U/ml Sagent Pharmaceutical Cat#25021040030 D-Sucrose Fisher Bioreagent Cat#BP22010 Sodium chloride anhydrous Sigma Cat#746398 Sodium bicarbonate Sigma Cat#S6297-250G Potassium chloride Sigma Cat#P3911-500G Sodium phosphate monobasic Fisher Bioreagent Cat#BP329-500 Calcium chloride dihydrate Sigma Cat#C3881-500G Magnesium sulfate Sigma Cat#M7506-500G Magnesium chloride Sigma Cat#M0250-500G Sodium pyruvate Sigma Cat#P2256-100G D-(+)-glucose Sigma Cat#G7528-250G Sodium L-ascorbic acid Sigma Cat#A4034-100G Cesium chloride Sigma Cat#C3032-25G Cesium methanesulfonate Sigma Cat#C1426-5G Cesium hydroxide Sigma Cat#232041-10G Ethylene glycol-bis(b-aminoethyl ether)N,N,N’,N’,-tetraacetic acid Sigma Cat#E3889-25G Tetraethylammonium chloride Sigma Cat#T2265-25G HEPES Sigma Cat#H4034-100G Adenosine 5’-triphosphate magnesium salt Sigma Cat#A9187-5x1G Guanosine 5’-triphosphate sodium salt hydrate Sigma Cat#G8877-100MG Qx-314-Cl Tocris Cat#231350 Potassium D-gluconate Sigma Cat#4500-100G Phosphocreatine disodium salt hydrate Sigma Cat#P7936-5G Tetrodotoxin Cayman Cat#14964 D-APV Tocris Cat#HB0225 NBQX Tocris Cat#0373-50MG (Continued on next page) ll OPEN ACCESS Cell 188, 1–15.e1–e11, May 15, 2025 e2 Please cite this article in press as: Kim et al., Meningeal lymphatics-microglia axis regulates synaptic physiology, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.02.022 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
Recombinant:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit-anti-GABA A receptor alpha1 Alomone Labs Cat#AGA001; RRID: AB_2756618 Mouse-anti-GAPDH ThermoFisher MA5-15738; RRID: AB_2537652 Rat-anti-a-tubulin Abcam Cat#ab6160; RRID: AB_305328 Rabbit-anti-GFAP Sigma Cat#SAB5600060 Rabbit-anti-Connexin-43 Abcam Cat#ab11370; RRID: AB_297976 Mouse-anti-c-fos Abcam Cat#ab208942; RRID: AB_2747772 Rabbit-anti-H3K9me3 Abcam Catab8898; RRID: AB_306848 Bacterial and virus strains AAV1-CMV-EGFP Vectorbiolab Cat#AAV-7002 AAV1-CMV-VEGF-C Vectorbiolab Cat#AAV-275994 AAV9-GAD67-InhiPre Lee et al.47 N/A AAV9-VEGFR3(Ig1-3)-Fc Song et al.34 N/A AAV9-VEGFR3(Ig4-7)-Fc Song et al.34 N/A Chemicals, peptides, and recombinant proteins 1200 mg/kg PLX5622-containing food chow Plexxikon Cat#D11100404I Control food chow for PLX5622 treatment Plexxikon Cat#D10001i Mouse recombininant IL-6 R&D systems Cat#40-6ML-200CF Ovalbumin Hooke Laboratories Inc. Cat#DSD0142 Soluble gp130-Fc R&D systems Cat#468-MG-100 Sterile artificial CSF Tocris Cat#3525-25ML Heparin sodium, porcine 1000 U/ml Sagent Pharmaceutical Cat#25021040030 D-Sucrose Fisher Bioreagent Cat#BP22010 Sodium chloride anhydrous Sigma Cat#746398 Sodium bicarbonate Sigma Cat#S6297-250G Potassium chloride Sigma Cat#P3911-500G Sodium phosphate monobasic Fisher Bioreagent Cat#BP329-500 Calcium chloride dihydrate Sigma Cat#C3881-500G Magnesium sulfate Sigma Cat#M7506-500G Magnesium chloride Sigma Cat#M0250-500G Sodium pyruvate Sigma Cat#P2256-100G D-(+)-glucose Sigma Cat#G7528-250G Sodium L-ascorbic acid Sigma Cat#A4034-100G Cesium chloride Sigma Cat#C3032-25G Cesium methanesulfonate Sigma Cat#C1426-5G Cesium hydroxide Sigma Cat#232041-10G Ethylene glycol-bis(b-aminoethyl ether)N,N,N’,N’,-tetraacetic acid Sigma Cat#E3889-25G Tetraethylammonium chloride Sigma Cat#T2265-25G HEPES Sigma Cat#H4034-100G Adenosine 5’-triphosphate magnesium salt Sigma Cat#A9187-5x1G Guanosine 5’-triphosphate sodium salt hydrate Sigma Cat#G8877-100MG Qx-314-Cl Tocris Cat#231350 Potassium D-gluconate Sigma Cat#4500-100G Phosphocreatine disodium salt hydrate Sigma Cat#P7936-5G Tetrodotoxin Cayman Cat#14964 D-APV Tocris Cat#HB0225 NBQX Tocris Cat#0373-50MG (Continued on next page) ll OPEN ACCESS Cell 188, 1–15.e1–e11, May 15, 2025 e2 Please cite this article in press as: Kim et al., Meningeal lymphatics-microglia axis regulates synaptic physiology, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.02.022 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
Control:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit-anti-GABA A receptor alpha1 Alomone Labs Cat#AGA001; RRID: AB_2756618 Mouse-anti-GAPDH ThermoFisher MA5-15738; RRID: AB_2537652 Rat-anti-a-tubulin Abcam Cat#ab6160; RRID: AB_305328 Rabbit-anti-GFAP Sigma Cat#SAB5600060 Rabbit-anti-Connexin-43 Abcam Cat#ab11370; RRID: AB_297976 Mouse-anti-c-fos Abcam Cat#ab208942; RRID: AB_2747772 Rabbit-anti-H3K9me3 Abcam Catab8898; RRID: AB_306848 Bacterial and virus strains AAV1-CMV-EGFP Vectorbiolab Cat#AAV-7002 AAV1-CMV-VEGF-C Vectorbiolab Cat#AAV-275994 AAV9-GAD67-InhiPre Lee et al.47 N/A AAV9-VEGFR3(Ig1-3)-Fc Song et al.34 N/A AAV9-VEGFR3(Ig4-7)-Fc Song et al.34 N/A Chemicals, peptides, and recombinant proteins 1200 mg/kg PLX5622-containing food chow Plexxikon Cat#D11100404I Control food chow for PLX5622 treatment Plexxikon Cat#D10001i Mouse recombininant IL-6 R&D systems Cat#40-6ML-200CF Ovalbumin Hooke Laboratories Inc. Cat#DSD0142 Soluble gp130-Fc R&D systems Cat#468-MG-100 Sterile artificial CSF Tocris Cat#3525-25ML Heparin sodium, porcine 1000 U/ml Sagent Pharmaceutical Cat#25021040030 D-Sucrose Fisher Bioreagent Cat#BP22010 Sodium chloride anhydrous Sigma Cat#746398 Sodium bicarbonate Sigma Cat#S6297-250G Potassium chloride Sigma Cat#P3911-500G Sodium phosphate monobasic Fisher Bioreagent Cat#BP329-500 Calcium chloride dihydrate Sigma Cat#C3881-500G Magnesium sulfate Sigma Cat#M7506-500G Magnesium chloride Sigma Cat#M0250-500G Sodium pyruvate Sigma Cat#P2256-100G D-(+)-glucose Sigma Cat#G7528-250G Sodium L-ascorbic acid Sigma Cat#A4034-100G Cesium chloride Sigma Cat#C3032-25G Cesium methanesulfonate Sigma Cat#C1426-5G Cesium hydroxide Sigma Cat#232041-10G Ethylene glycol-bis(b-aminoethyl ether)N,N,N’,N’,-tetraacetic acid Sigma Cat#E3889-25G Tetraethylammonium chloride Sigma Cat#T2265-25G HEPES Sigma Cat#H4034-100G Adenosine 5’-triphosphate magnesium salt Sigma Cat#A9187-5x1G Guanosine 5’-triphosphate sodium salt hydrate Sigma Cat#G8877-100MG Qx-314-Cl Tocris Cat#231350 Potassium D-gluconate Sigma Cat#4500-100G Phosphocreatine disodium salt hydrate Sigma Cat#P7936-5G Tetrodotoxin Cayman Cat#14964 D-APV Tocris Cat#HB0225 NBQX Tocris Cat#0373-50MG (Continued on next page) ll OPEN ACCESS Cell 188, 1–15.e1–e11, May 15, 2025 e2 Please cite this article in press as: Kim et al., Meningeal lymphatics-microglia axis regulates synaptic physiology, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.02.022 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
Sterility:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit-anti-GABA A receptor alpha1 Alomone Labs Cat#AGA001; RRID: AB_2756618 Mouse-anti-GAPDH ThermoFisher MA5-15738; RRID: AB_2537652 Rat-anti-a-tubulin Abcam Cat#ab6160; RRID: AB_305328 Rabbit-anti-GFAP Sigma Cat#SAB5600060 Rabbit-anti-Connexin-43 Abcam Cat#ab11370; RRID: AB_297976 Mouse-anti-c-fos Abcam Cat#ab208942; RRID: AB_2747772 Rabbit-anti-H3K9me3 Abcam Catab8898; RRID: AB_306848 Bacterial and virus strains AAV1-CMV-EGFP Vectorbiolab Cat#AAV-7002 AAV1-CMV-VEGF-C Vectorbiolab Cat#AAV-275994 AAV9-GAD67-InhiPre Lee et al.47 N/A AAV9-VEGFR3(Ig1-3)-Fc Song et al.34 N/A AAV9-VEGFR3(Ig4-7)-Fc Song et al.34 N/A Chemicals, peptides, and recombinant proteins 1200 mg/kg PLX5622-containing food chow Plexxikon Cat#D11100404I Control food chow for PLX5622 treatment Plexxikon Cat#D10001i Mouse recombininant IL-6 R&D systems Cat#40-6ML-200CF Ovalbumin Hooke Laboratories Inc. Cat#DSD0142 Soluble gp130-Fc R&D systems Cat#468-MG-100 Sterile artificial CSF Tocris Cat#3525-25ML Heparin sodium, porcine 1000 U/ml Sagent Pharmaceutical Cat#25021040030 D-Sucrose Fisher Bioreagent Cat#BP22010 Sodium chloride anhydrous Sigma Cat#746398 Sodium bicarbonate Sigma Cat#S6297-250G Potassium chloride Sigma Cat#P3911-500G Sodium phosphate monobasic Fisher Bioreagent Cat#BP329-500 Calcium chloride dihydrate Sigma Cat#C3881-500G Magnesium sulfate Sigma Cat#M7506-500G Magnesium chloride Sigma Cat#M0250-500G Sodium pyruvate Sigma Cat#P2256-100G D-(+)-glucose Sigma Cat#G7528-250G Sodium L-ascorbic acid Sigma Cat#A4034-100G Cesium chloride Sigma Cat#C3032-25G Cesium methanesulfonate Sigma Cat#C1426-5G Cesium hydroxide Sigma Cat#232041-10G Ethylene glycol-bis(b-aminoethyl ether)N,N,N’,N’,-tetraacetic acid Sigma Cat#E3889-25G Tetraethylammonium chloride Sigma Cat#T2265-25G HEPES Sigma Cat#H4034-100G Adenosine 5’-triphosphate magnesium salt Sigma Cat#A9187-5x1G Guanosine 5’-triphosphate sodium salt hydrate Sigma Cat#G8877-100MG Qx-314-Cl Tocris Cat#231350 Potassium D-gluconate Sigma Cat#4500-100G Phosphocreatine disodium salt hydrate Sigma Cat#P7936-5G Tetrodotoxin Cayman Cat#14964 D-APV Tocris Cat#HB0225 NBQX Tocris Cat#0373-50MG (Continued on next page) ll OPEN ACCESS Cell 188, 1–15.e1–e11, May 15, 2025 e2 Please cite this article in press as: Kim et al., Meningeal lymphatics-microglia axis regulates synaptic physiology, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.02.022 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
Clinical Proteomics:Article Title: Meningeal lymphatics-microglia axis regulates synaptic physiology.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit-anti-GABA A receptor alpha1 Alomone Labs Cat#AGA001; RRID: AB_2756618 Mouse-anti-GAPDH ThermoFisher MA5-15738; RRID: AB_2537652 Rat-anti-a-tubulin Abcam Cat#ab6160; RRID: AB_305328 Rabbit-anti-GFAP Sigma Cat#SAB5600060 Rabbit-anti-Connexin-43 Abcam Cat#ab11370; RRID: AB_297976 Mouse-anti-c-fos Abcam Cat#ab208942; RRID: AB_2747772 Rabbit-anti-H3K9me3 Abcam Catab8898; RRID: AB_306848 Bacterial and virus strains AAV1-CMV-EGFP Vectorbiolab Cat#AAV-7002 AAV1-CMV-VEGF-C Vectorbiolab Cat#AAV-275994 AAV9-GAD67-InhiPre Lee et al.47 N/A AAV9-VEGFR3(Ig1-3)-Fc Song et al.34 N/A AAV9-VEGFR3(Ig4-7)-Fc Song et al.34 N/A Chemicals, peptides, and recombinant proteins 1200 mg/kg PLX5622-containing food chow Plexxikon Cat#D11100404I Control food chow for PLX5622 treatment Plexxikon Cat#D10001i Mouse recombininant IL-6 R&D systems Cat#40-6ML-200CF Ovalbumin Hooke Laboratories Inc. Cat#DSD0142 Soluble gp130-Fc R&D systems Cat#468-MG-100 Sterile artificial CSF Tocris Cat#3525-25ML Heparin sodium, porcine 1000 U/ml Sagent Pharmaceutical Cat#25021040030 D-Sucrose Fisher Bioreagent Cat#BP22010 Sodium chloride anhydrous Sigma Cat#746398 Sodium bicarbonate Sigma Cat#S6297-250G Potassium chloride Sigma Cat#P3911-500G Sodium phosphate monobasic Fisher Bioreagent Cat#BP329-500 Calcium chloride dihydrate Sigma Cat#C3881-500G Magnesium sulfate Sigma Cat#M7506-500G Magnesium chloride Sigma Cat#M0250-500G Sodium pyruvate Sigma Cat#P2256-100G D-(+)-glucose Sigma Cat#G7528-250G Sodium L-ascorbic acid Sigma Cat#A4034-100G Cesium chloride Sigma Cat#C3032-25G Cesium methanesulfonate Sigma Cat#C1426-5G Cesium hydroxide Sigma Cat#232041-10G Ethylene glycol-bis(b-aminoethyl ether)N,N,N’,N’,-tetraacetic acid Sigma Cat#E3889-25G Tetraethylammonium chloride Sigma Cat#T2265-25G HEPES Sigma Cat#H4034-100G Adenosine 5’-triphosphate magnesium salt Sigma Cat#A9187-5x1G Guanosine 5’-triphosphate sodium salt hydrate Sigma Cat#G8877-100MG Qx-314-Cl Tocris Cat#231350 Potassium D-gluconate Sigma Cat#4500-100G Phosphocreatine disodium salt hydrate Sigma Cat#P7936-5G Tetrodotoxin Cayman Cat#14964 D-APV Tocris Cat#HB0225 NBQX Tocris Cat#0373-50MG (Continued on next page) ll OPEN ACCESS Cell 188, 1–15.e1–e11, May 15, 2025 e2 Please cite this article in press as: Kim et al., Meningeal lymphatics-microglia axis regulates synaptic physiology, Cell (2025), https://doi.org/ 10.1016/j.cell.2025.02.022 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Picrotoxin Abcam Cat#Ab120315 Borosilicate glass capillary Harvard Apparatus Cat#23-30-0065 4’,6-diamidino-2-phenylindole Sigma Cat#D9542 TrueBlack Lipofuscin autofluorescence quencher Biotium Cat#23007 Tissue-Plus O.C.T.
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